Freezing Medium (Serum-free & animal origin-free)

Product detailed description

In in vitro cell culture, in order to preserve the biological activity of cells for a long time, the cells must be cryopreserved, and resuscitated when necessary. At present, the most commonly used method for cell cryopreservation is liquid nitrogen method. In the process, add an appropriate amount of protective agent to slowly cool the cells to the specified temperature range, so as to achieve the purpose of protecting the cells. The Serum-free Cell Freezing Medium is a special cryopreservation product developed by Elabscience-Pricella for hundreds of types of cells by continuously optimizing experimental conditions for cell cryopreservation and resuscitation in the long-term cell research. The product adds cell sedimentation stabilizer, which can delay the sedimentation rate of cells in the process of cryopreservation, prevent cells from squeezing each other and affect the effect of cell cryopreservation. In addition, a variety of cryoprotectants, such as cell membrane protectors, permeable intracellular membrane protectors, and non-permeable cell protectors, are added. These components combine with water molecules in the solution to hydrate and weaken the crystallization process of water, increasing the viscosity of the solution and reducing the formation of ice crystals, which can greatly reduce the damage of ice crystals to cells during cryopreservation, and effectively improve the survival rate of cell after resuscitation. The product formula is clear, does not contain serum or animal derived protein, which can reduce comtamination risk from bacteria, viruses and mycoplasma, and ensure the safety of frozen cells. It is not only suitable for conventional cell lines, primary cells, but also for serum-free culture cells and protein-expressing cells. Compared with traditional cryopreservation media, there is no need for tedious and time-consuming gradient freezing steps, and no need for expensive program cooling equipment. Cells can be directly resuspended and placed at -80°C, and transferred to liquid nitrogen the next day to complete the entire process, saving a lot of time and energy.

• This product is for research use only
• This product is sterilized by 0.1μm filtration
• It is necessary to pay attention to the aseptic operation and avoid the contamination
• Avoid repeated freezing and thawing. It is recommended to defrost at 2-8°C and aliquot the solution and store at -20°C
• It is not recommended to store this product at room temperature for a long time
• Since this product contains DMSO, please take protective measures to avoid direct contact with skin